Difference between revisions of "Part:BBa K4385014:Design"

 
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===Design Notes===
 
===Design Notes===
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The part can enhance the tolerance of engineering bacteria to heavy metal solutions, and can be used as a supplement for surface display to improve the adsorption efficiency of engineering bacteria to heavy metal ions.
 
  
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The part mainly consists of the constitutive promoter (BBa_J23100), the lead-inducible regulatory protein PbrR (BBa_K4385000), the outer membrane protein A(BBa_K4385002) and the lipoprotein of E. coli(BBa_K4385001).
  
  

Revision as of 15:45, 6 October 2022


Lead constitutive surface display


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 938
    Illegal PstI site found at 880
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1120
    Illegal NheI site found at 1143
    Illegal SpeI site found at 938
    Illegal PstI site found at 880
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 938
    Illegal PstI site found at 880
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 938
    Illegal PstI site found at 880
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 671


Design Notes

The part mainly consists of the constitutive promoter (BBa_J23100), the lead-inducible regulatory protein PbrR (BBa_K4385000), the outer membrane protein A(BBa_K4385002) and the lipoprotein of E. coli(BBa_K4385001).


Source

It comes from E. coli genomic DNA.

References