Difference between revisions of "Part:BBa K4385014"

Revision as of 15:41, 6 October 2022

Lead constitutive surface display

Lead constitutive surface display consists of the constitutive promoter J23100, the lead-inducible regulatory protein PbrR and the anchoring protein Lpp-OmpA. J23100 continuously expresses PbrR for lead ion adsorption, and Lpp-OmpA is used as an anchoring protein to anchor PbrR on the surface of E. coli to construct a whole-cell biosorption device for lead.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 938
Illegal PstI site found at 880
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1120
Illegal NheI site found at 1143
Illegal SpeI site found at 938
Illegal PstI site found at 880
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1255
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 938
Illegal PstI site found at 880
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 938
Illegal PstI site found at 880
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 671

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 <partinfo>BBa_K4385014 short</partinfo>
-The part can enhance the tolerance of engineering bacteria to heavy metal solutions, and can be used as a supplement for surface display to improve the adsorption efficiency of engineering bacteria to heavy metal ions.
+Lead constitutive surface display consists of the constitutive promoter J23100, the lead-inducible regulatory protein PbrR and the anchoring protein Lpp-OmpA. J23100 continuously expresses PbrR for lead ion adsorption, and Lpp-OmpA is used as an anchoring protein to anchor PbrR on the surface of E. coli to construct a whole-cell biosorption device for lead.
 <!-- Add more about the biology of this part here