Difference between revisions of "Part:BBa K4385012"

Revision as of 14:18, 6 October 2022

Copper sensor

The copper ion sensor part is composed of PcusC and CusSR two-component membrane associated sensor kinase system. CusSR is composed of membrane-associated histidine kinase CusS and transcription activator CusR, responsible for sensing the presence of external Cu2+. When external copper is sensed, the transmembrane structure of CusS protein undergoes a conformational change, and at the same time, autophosphorylation occurs to transmit the signal into the cell. The phosphoryl group is then transferred to the CusR protein, triggering a conformational change in CusR, and the phosphoryl group binds to a sequence in front of the PcusC promoter to turn on downstream gene expression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 826

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4385012 short</partinfo>
-This part is composed of ZraSR two-component signal transport system, PzraP and OmpC-ZBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PzraP promoter promotes the transcription of downstream OmpC-ZBP, and realizes the adsorption of zinc ions in the environment. Add 6*Hig Tag to this sequence to purify recombinant protein.
+The copper ion sensor part is composed of PcusC and CusSR two-component membrane associated sensor kinase system. CusSR is composed of membrane-associated histidine kinase CusS and transcription activator CusR, responsible for sensing the presence of external Cu2+. When external copper is sensed, the transmembrane structure of CusS protein undergoes a conformational change, and at the same time, autophosphorylation occurs to transmit the signal into the cell. The phosphoryl group is then transferred to the CusR protein, triggering a conformational change in CusR, and the phosphoryl group binds to a sequence in front of the PcusC promoter to turn on downstream gene expression.
 <!-- Add more about the biology of this part here