Difference between revisions of "Part:BBa K4385011:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This part is composed of CusSR two-component signal transport system, PcusC and OmpC-CBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PcusC  promotes the transcription of downstream OmpC-CBP, and realizes the adsorption of copper ions in the environment. The 6*His Tag was added to this sequence to purify the recombinant protein.
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The lead sensor is composed of BBa_K4385009 pbr and BBa_E0040 GFP. Note the order of inducible promoter Pbc, constitutive promoter Pbr and lead-sensing regulatory protein PbrR in pbr.
  
 
===Source===
 
===Source===

Revision as of 14:15, 6 October 2022


Lead sensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 462
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 462
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 462
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 253
    Illegal BsaI.rc site found at 1394


Design Notes

The lead sensor is composed of BBa_K4385009 pbr and BBa_E0040 GFP. Note the order of inducible promoter Pbc, constitutive promoter Pbr and lead-sensing regulatory protein PbrR in pbr.

Source

It is amplified from E. coli genomic DNA.

References