Difference between revisions of "Part:BBa K4385011"

Revision as of 14:09, 6 October 2022

Lead sensor

The lead sensor is composed of lead-inducible promoter and GFP. When there is no lead ion in the environment, PbrR protein is expressed in the presence of Pbr constitutive promoter and binds to its downstream inducible promoter Pbc to repress GFP expression; when lead ion reaches a certain concentration in the environment, PbrR protein chelates lead ion so that it cannot bind to the inducible promoter Pbc and downstream GFP can be expressed and the engineered bacteria can see green fluorescence.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 462
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 462
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 462
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 462
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 253
Illegal BsaI.rc site found at 1394

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 The lead sensor is composed of lead-inducible promoter and GFP. When there is no lead ion in the environment, PbrR protein is expressed in the presence of Pbr constitutive promoter and binds to its downstream inducible promoter Pbc to repress GFP expression; when lead ion reaches a certain concentration in the environment, PbrR protein chelates lead ion so that it cannot bind to the inducible promoter Pbc and downstream GFP can be expressed and the engineered bacteria can see green fluorescence.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===