Difference between revisions of "Part:BBa K176157"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K176157 short</partinfo> | <partinfo>BBa_K176157 short</partinfo> | ||
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Add LVA degradation tag (widely used in C0040, C0051, C0061...) to ccdB(K145151) C-terminal: | Add LVA degradation tag (widely used in C0040, C0051, C0061...) to ccdB(K145151) C-terminal: | ||
− | [K145151 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA] | + | [K145151 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA] |
− | + | A A N D E N Y A L V A | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 13:37, 14 September 2009
ccdB-LVA coding sequence
Add LVA degradation tag (widely used in C0040, C0051, C0061...) to ccdB(K145151) C-terminal:
[K145151 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA] A A N D E N Y A L V A
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 216