Difference between revisions of "Part:BBa K4368001"
Line 8: | Line 8: | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | |||
+ | ==Contribution == | ||
+ | *'''Group:''' [https://2022.igem.wiki/uma-malaga/index.html] | ||
+ | *'''Author:''' Molina Calvo, Alonso | ||
<!-- --> | <!-- --> |
Revision as of 09:24, 6 October 2022
pcstA + rbs + yebF + cex + terminator
Cex encodes for the exoglucanase gene of Celullomonas fimi (BBa_K118022). This enzyme is responsible of the degradation of celullose working coordinated with th genes cenA and bglX. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by glucose concentration (BBa_K118011). Furthermore, we improve this composite adding a gen encoding a motor protein named YebF (BBa_K1610300) that secrete the enzyme out of the E. coli membrane. The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1044
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 268
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 1050
Illegal NgoMIV site found at 1552 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1097
Illegal SapI.rc site found at 1180