Difference between revisions of "Part:BBa K4425002"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4425002 short</partinfo>
 
<partinfo>BBa_K4425002 short</partinfo>
  
−
a glucosyltransferase
+
a glucosyltransferase that can introduce a reversible protecting glucose group into indoxyl, turning low water solubility product indigo (or tyrian purple) to soluble indican.
 +
[[Image:25001-2.png|thumbnail|200px|center|'''Figure 1:''' PtUGT can convert indoxyl to indican ]]
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
gene of PtUGT and FMO was assembled with pET28a backbone to construct pET28a-PtUGT-FMO. PtUGT and FMO were co-expression to convert indole (or 6-Br-indole) to indican which gives a sustainable indigo dyeing strategy.
 +
[[Image:25002-1.png|thumbnail|400px|center|'''Figure 2:''' schematic of pET28a-PtUGT-FMO ]]
 +
 +
==Source==
 +
PtUGT come from <i>P. tinctorium</i>
  
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4425002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4425002 SequenceAndFeatures</partinfo>
Line 17: Line 21:
 
<partinfo>BBa_K4425002 parameters</partinfo>
 
<partinfo>BBa_K4425002 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
==Reference==
 +
Hsu, T. M., Welner, D. H., Russ, Z. N., Cervantes, B., Prathuri, R. L., Adams, P. D., & Dueber, J. E. Employing a biochemical protecting group for a sustainable indigo dyeing strategy. Nature chemical biology, 2018, 14(3), 256–261.

Latest revision as of 09:07, 6 October 2022


PtUGT

a glucosyltransferase that can introduce a reversible protecting glucose group into indoxyl, turning low water solubility product indigo (or tyrian purple) to soluble indican.

Figure 1: PtUGT can convert indoxyl to indican

Usage and Biology

gene of PtUGT and FMO was assembled with pET28a backbone to construct pET28a-PtUGT-FMO. PtUGT and FMO were co-expression to convert indole (or 6-Br-indole) to indican which gives a sustainable indigo dyeing strategy.

Figure 2: schematic of pET28a-PtUGT-FMO

Source

PtUGT come from P. tinctorium

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 886
    Illegal AgeI site found at 1093
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 484


Reference

Hsu, T. M., Welner, D. H., Russ, Z. N., Cervantes, B., Prathuri, R. L., Adams, P. D., & Dueber, J. E. Employing a biochemical protecting group for a sustainable indigo dyeing strategy. Nature chemical biology, 2018, 14(3), 256–261.