Difference between revisions of "Part:BBa K4425001"
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<partinfo>BBa_K4425001 short</partinfo> | <partinfo>BBa_K4425001 short</partinfo> | ||
| − | a β-glucosidase | + | a β-glucosidase that can remove the protecting glucose from indican, and convert indican to indoxyl. |
| + | [[Image:25001-2.png|thumbnail|200px|center|'''Figure 1:''' BGL can convert indican to indoxyl.]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | BGL was assemble with pET28a backbone to construct pET28a-BGL. BGL is induced to express to convert soluble indican to indoxyl, and the lattet can turn to indigo (or tyrian purple) by spontaneous oxidation | ||
| + | [[Image:25001-1.png|thumbnail|400px|center|'''Figure 2:''' schematic of pET28a-BGL.]] | ||
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| + | ==Source== | ||
| + | BGL comes from <i>P. tinctorium</i> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4425001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4425001 SequenceAndFeatures</partinfo> | ||
Latest revision as of 08:54, 6 October 2022
BGL
a β-glucosidase that can remove the protecting glucose from indican, and convert indican to indoxyl.
Usage and Biology
BGL was assemble with pET28a backbone to construct pET28a-BGL. BGL is induced to express to convert soluble indican to indoxyl, and the lattet can turn to indigo (or tyrian purple) by spontaneous oxidation
Source
BGL comes from P. tinctorium
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal XhoI site found at 1423 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal AgeI site found at 334 - 1000COMPATIBLE WITH RFC[1000]