Difference between revisions of "Part:BBa K4122017"
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| + | <p>GFP+ and RFP+ suggested the successful construction of Spycatcher/Spytag system and Snoopcatcher/Snooptag system.</p> | ||
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| + | <br/><b>Fig.1</b> The fluorescence result of the spy/snoop tag and catcher system. | ||
| + | <p>A and D, bright field; B and E, Green fluorescence; C and F, Red fluorescence</p> | ||
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Revision as of 08:04, 6 October 2022
SP-GFP-His-Spytag+SP-RFP-cMyc-Snooptag+SP-CBM-SC-SC-SNC-SC-V5-7813
SP-GFP-His-Spytag+ SP-RFP-cMyc-Snooptag+ SP-CBM-SC-SC-SNC-SC-V5-7813
This part is composed of three parts:
Part 1:SP-GFP-His-Spytag BBa_K4122014
Part 2:SP-RFP-cMyc-Snooptag BBa_K4122015
Part 3:SP-CBM-SC-SC-SNC-SC-V5-7813 BBa_K4122016
Characterization-Introduction of Tag-Catcher system to co-display PETase and MHETase
To attain co-display, we combined our display system with two selective protein binding systems, SpyTag-SpyCatcher and SnoopTag-SnoopCatcher. In our experiment, GFP and RFP were used to indicate the successful construction of Spycatcher/Spytag and Snoopcatcher/Snooptag systems. We initially tried two catcher types with a ratio of 1:3.
Fig.1 The construction of plasmid Ts-PGAPDH--TENO1A, the surface display system for displaying both GFP and RFP. (BBa_K4122017)
SC: Spycatcher BBa_K4122008; SNC: Snoopcatcher BBa_K4122010; V5: V5 tag BBa_K3829004; CBM: carbohydrate binding domain BBa_K4122006
GFP+ and RFP+ suggested the successful construction of Spycatcher/Spytag system and Snoopcatcher/Snooptag system.
Fig.1 The fluorescence result of the spy/snoop tag and catcher system.
A and D, bright field; B and E, Green fluorescence; C and F, Red fluorescence
Sequence and Features