Difference between revisions of "Part:BBa K4122017"

Revision as of 06:55, 6 October 2022

SP-GFP-His-Spytag+SP-RFP-cMyc-Snooptag+SP-CBM-SC-SC-SNC-SC-V5-7813

SP-GFP-His-Spytag+ SP-RFP-cMyc-Snooptag+ SP-CBM-SC-SC-SNC-SC-V5-7813

This part is composed of three parts:

Part 1:SP-GFP-His-Spytag <a href="https://parts.igem.org/Part:BBa_K4122014"> BBa_K4122014</a>

Part 2:SP-RFP-cMyc-Snooptag <a href="https://parts.igem.org/Part:BBa_K4122015"> BBa_K4122015</a>

Part 3:SP-CBM-SC-SC-SNC-SC-V5-7813 <a href="https://parts.igem.org/Part:BBa_K4122016"> BBa_K4122016</a>

Characterization-Introduction of Tag-Catcher system to co-display PETase and MHETase

To attain co-display, we combined our display system with two selective protein binding systems, SpyTag-SpyCatcher and SnoopTag-SnoopCatcher. In our experiment, GFP and RFP were used to indicate the successful construction of Spycatcher/Spytag and Snoopcatcher/Snooptag systems. We initially tried two catcher types with a ratio of 1:3.

<img src="" style = "width:45%;">
Fig.1 The construction of plasmid Ts-PGAPDH--TENO1A, the surface display system for displaying both GFP and RFP. (<a href="https://parts.igem.org/Part:BBa_K4122017">BBa_K4122017</a>)

SC: Spycatcher BBa_K4122008; SNC: Snoopcatcher BBa_K4122010; V5: V5 tag BBa_K3829004; CBM: carbohydrate binding domain BBa_K4122006

Sequence and Features