Difference between revisions of "Part:BBa K4122017"
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<p>This part is composed of three parts:
 
<p>This part is composed of three parts:
 
</p>
 
</p>
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<p>Part 1:SP-GFP-His-Spytag</p>
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<p>Part 1:SP-GFP-His-Spytag <a href="https://parts.igem.org/Part:BBa_K4122014">BBa_K4122014</a></p>
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<p>Part 2:SP-RFP-cMyc-Snooptag</p>
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<p>Part 2:SP-RFP-cMyc-Snooptag <a href="https://parts.igem.org/Part:BBa_K4122015">BBa_K4122015</a></p>
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<p>Part 3:SP-CBM-SC-SC-SNC-SC-V5-7813</p>
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<p>Part 3:SP-CBM-SC-SC-SNC-SC-V5-7813 <a href="https://parts.igem.org/Part:BBa_K4122016">BBa_K4122016</a></p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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Introduction of Tag-Catcher system to co-display PETase and MHETase
 
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To attain co-display, we combined our display system with two selective protein binding systems, SpyTag-SpyCatcher and SnoopTag-SnoopCatcher.
 
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In our experiment, GFP and RFP were used to indicate the successful construction of Spycatcher/Spytag and Snoopcatcher/Snooptag systems. We initially tried two catcher types with a ratio of 1:3.
 
  
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<h3>Characterization-Introduction of Tag-Catcher system to co-display PETase and MHETase</h3>
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<P>To attain co-display, we combined our display system with two selective protein binding systems, SpyTag-SpyCatcher and SnoopTag-SnoopCatcher.
 +
In our experiment, GFP and RFP were used to indicate the successful construction of Spycatcher/Spytag and Snoopcatcher/Snooptag systems. We initially tried two catcher types with a ratio of 1:3.</P>
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<img src="https://static.igem.wiki/teams/4122/wiki/contribution/contribution-of-gfp-patial-zoe.png" style = "width:45%;">
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<br/><b>Fig.1</b> The construction of plasmid Ts-PGAPDH--TENO1A, the surface display system for displaying both GFP and RFP. (<a href="https://parts.igem.org/Part:BBa_K4122017">BBa_K4122017</a>)
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<p>SC: Spycatcher BBa_K4122008; SNC: Snoopcatcher BBa_K4122010; V5: V5 tag BBa_K3829004; CBM: carbohydrate binding domain BBa_K4122006</p>
  
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<br/>
  
  

Revision as of 06:54, 6 October 2022


SP-GFP-His-Spytag+SP-RFP-cMyc-Snooptag+SP-CBM-SC-SC-SNC-SC-V5-7813

SP-GFP-His-Spytag+ SP-RFP-cMyc-Snooptag+ SP-CBM-SC-SC-SNC-SC-V5-7813

This part is composed of three parts:

Part 1:SP-GFP-His-Spytag <a href="https://parts.igem.org/Part:BBa_K4122014">BBa_K4122014</a>

Part 2:SP-RFP-cMyc-Snooptag <a href="https://parts.igem.org/Part:BBa_K4122015">BBa_K4122015</a>

Part 3:SP-CBM-SC-SC-SNC-SC-V5-7813 <a href="https://parts.igem.org/Part:BBa_K4122016">BBa_K4122016</a>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]