Difference between revisions of "Part:BBa K4122017"

Revision as of 06:47, 6 October 2022

SP-GFP-His-Spytag+SP-RFP-cMyc-Snooptag+SP-CBM-SC-SC-SNC-SC-V5-7813

SP-GFP-His-Spytag+ SP-RFP-cMyc-Snooptag+ SP-CBM-SC-SC-SNC-SC-V5-7813

This part is composed of three parts:

Part 1:SP-GFP-His-Spytag

Part 2:SP-RFP-cMyc-Snooptag

Part 3:SP-CBM-SC-SC-SNC-SC-V5-7813

Sequence and Features

Assembly Compatibility:

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4122017 short</partinfo>
-SP-GFP-His-Spytag+SP-RFP-cMyc-Snooptag+SP-CBM-SC-SC-SNC-SC-V5-7813
+SP-GFP-His-Spytag+ SP-RFP-cMyc-Snooptag+ SP-CBM-SC-SC-SNC-SC-V5-7813
-Part 1&#65306;SP-GFP-His-Spytag
-Part 2&#65306;SP-RFP-cMyc-Snooptag
-Part 3&#65306;SP-CBM-SC-SC-SNC-SC-V5-7813
+<p>This part is composed of three parts:
+</p>
+<p>Part 1:SP-GFP-His-Spytag</p>
+<p>Part 2:SP-RFP-cMyc-Snooptag</p>
+<p>Part 3:SP-CBM-SC-SC-SNC-SC-V5-7813</p>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+Introduction of Tag-Catcher system to co-display PETase and MHETase
+To attain co-display, we combined our display system with two selective protein binding systems, SpyTag-SpyCatcher and SnoopTag-SnoopCatcher.
+In our experiment, GFP and RFP were used to indicate the successful construction of Spycatcher/Spytag and Snoopcatcher/Snooptag systems. We initially tried two catcher types with a ratio of 1:3.
 <!-- -->