Difference between revisions of "Part:BBa K4122006"
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<p>The carbohydrate-binding domain can guide cellulosomes to adsorb on the surface of crystalline cellulose or hemicellulose molecules through specific interactions, increase the local substrate concentration of cellulosomes, improve mass transfer efficiency, and improve degradation efficiency.</p> | <p>The carbohydrate-binding domain can guide cellulosomes to adsorb on the surface of crystalline cellulose or hemicellulose molecules through specific interactions, increase the local substrate concentration of cellulosomes, improve mass transfer efficiency, and improve degradation efficiency.</p> | ||
Revision as of 05:48, 6 October 2022
CBM
The carbohydrate-binding domain can guide cellulosomes to adsorb on the surface of crystalline cellulose or hemicellulose molecules through specific interactions, increase the local substrate concentration of cellulosomes, improve mass transfer efficiency, and improve degradation efficiency.
CBM has two functions:
1. hydrophobic properties for better secretion.
2. CBM can improve the catalytic efficiency of carbohydrate active enzymes.
When we introduced CBM in the original system, the first thing we needed to determine was whether CBM had an impact on the surface display system. The experimental results showed that the presence of CBM did not affect the expression of GFP and the localization of anchoring proteins, as well as the determination of immunofluorescence.
Fig.1 CBM did not affect basic function of the surface display system.
Sequence and Features