Difference between revisions of "Part:BBa K4463005"

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−	IGEM_XJTLU 2022 tested the function of ShMT3 by adding it with CsgA, which realized the surface display of ShMT3 on <i>E.coli</i>	+	IGEM_XJTLU 2022 tested the function of ShMT3 by adding it with CsgA, which realized the surface display of ShMT3 on <i>E.coli</i>.
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Revision as of 03:31, 6 October 2022

ShMT3

This is an improved part based on BBa_k4463001. The ability of Metallothioneins to bind metal ions depends on the amount of cysteine (Cys). Therefore, in order to improve the metal ion binding capacity of ShMT, we performed a three-point mutation of the original sequence by targeted mutagenesis to increase the Cys content^[1].

IGEM_XJTLU 2022 tested the function of ShMT3 by adding it with CsgA, which realized the surface display of ShMT3 on E.coli.

Figure1. E.coli expressed ShMT3 protein showed higher adsorption of Cu(II) in 1.8mM CuSO4 solution.
Figure2. E.coli expressed ShMT3 protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.

For more information about our measure methods, please visit our wiki.

Reference

1 X. Li, Z. Ren, M. J. C. Crabbe, L. Wang and W. Ma, Ecotoxicology and Environmental Safety, 2021, 222, 112512.  

Sequence and Features