Difference between revisions of "Part:BBa K4463006"
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CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of ShMT3, a protein belongs to Metallothionein family, achieved by linking to CsgA.
 
CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of ShMT3, a protein belongs to Metallothionein family, achieved by linking to CsgA.
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IGEM_XJTLU 2022 tested the function of CsgA-ShMT3 by measuring the concentration of adsorbed metal ions, such as Cu(II), Cd(II).
  
 
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Figure2. <i>E.coli</i> expressed ShMT3 protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.
 
Figure2. <i>E.coli</i> expressed ShMT3 protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.
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For more information about our measure methods, please visit our wiki.
  
 
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Revision as of 03:15, 6 October 2022


CsgA-ShMT3

CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of ShMT3, a protein belongs to Metallothionein family, achieved by linking to CsgA.
IGEM_XJTLU 2022 tested the function of CsgA-ShMT3 by measuring the concentration of adsorbed metal ions, such as Cu(II), Cd(II).

Figure1. E.coli expressed ShMT3 protein showed higher adsorption of Cu(II) in 1.8mM CuSO4 solution.
Figure2. E.coli expressed ShMT3 protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.

For more information about our measure methods, please visit our wiki.
 

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]