Difference between revisions of "Part:BBa K4321007:Design"

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===References===
 
===References===
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Soberón, M., López-Díaz, J. A., & Bravo, A. (2013). Cyt toxins produced by bacillus thuringiensis: A protein fold conserved in several pathogenic microorganisms. Peptides, 41, 87–93. https://doi.org/10.1016/j.peptides.2012.05.023
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Torres-Quintero, M.-C., Gómez, I., Pacheco, S., Sánchez, J., Flores, H., Osuna, J., Mendoza, G., Soberón, M., & Bravo, A. (2018). Engineering bacillus thuringiensis cyt1aa toxin specificity from dipteran to lepidopteran toxicity. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-22740-9
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Valtierra-de-Luis, D., Villanueva, M., Berry, C., & Caballero, P. (2020). Potential for bacillus thuringiensis and other bacterial toxins as biological control agents to combat dipteran pests of medical and agronomic importance. Toxins, 12(12), 773. https://doi.org/10.3390/toxins12120773

Revision as of 00:40, 6 October 2022


Cyt1Aa Cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 264
    Illegal PstI site found at 1006
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 264
    Illegal PstI site found at 1006
    Illegal NotI site found at 1888
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 264
    Illegal BglII site found at 1304
    Illegal BamHI site found at 683
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 264
    Illegal PstI site found at 1006
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 264
    Illegal PstI site found at 1006
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Unlike Cyt2Ba, the stability of the Cyt1Aa protein and colony formation in host and E.coli cells are reliant on the co-expression of a P20 helper protein. When trying to express Cyt1Aa, this factor should be considered.

Source

The cassette was synthesized by IDT. Information on the origin of each part can be found on their respective part pages: P20 helpher protein - BBa_K2938004, Cyt1Aa - BBa_K2938003, RBS - BBa_B0034, mScarlet - BBa_K4321004, Terminator - BBa_K4321008.

References

Soberón, M., López-Díaz, J. A., & Bravo, A. (2013). Cyt toxins produced by bacillus thuringiensis: A protein fold conserved in several pathogenic microorganisms. Peptides, 41, 87–93. https://doi.org/10.1016/j.peptides.2012.05.023

Torres-Quintero, M.-C., Gómez, I., Pacheco, S., Sánchez, J., Flores, H., Osuna, J., Mendoza, G., Soberón, M., & Bravo, A. (2018). Engineering bacillus thuringiensis cyt1aa toxin specificity from dipteran to lepidopteran toxicity. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-22740-9

Valtierra-de-Luis, D., Villanueva, M., Berry, C., & Caballero, P. (2020). Potential for bacillus thuringiensis and other bacterial toxins as biological control agents to combat dipteran pests of medical and agronomic importance. Toxins, 12(12), 773. https://doi.org/10.3390/toxins12120773