Difference between revisions of "Part:BBa K4165082"
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<partinfo>BBa_K4165082 short</partinfo>
 
<partinfo>BBa_K4165082 short</partinfo>
  
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This basic part encodes Human serine protease inhibitor known as SPINK4 which is able to  
+
This basic part encodes Human serine protease inhibitor known as SPINK9 which is able to inhibit trypsin-like proteases, like HtrA1 (BBa_K4165004).
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inhibit trypsin-like proteases, like HtrA1 (BBa_K4165004).
+
 
  
 
===Usage and Biology===
 
===Usage and Biology===
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This type of family encodes for a type of inhibitor that is able to inhibit serine proteases and it is predicted to be located extracellularly. The inhibitor binds to trypsin proteases and since the catalytic core of HtrA1 (BBa_K4165004) is considered as a tyrpsin-like catalytic domain, so this inhibitor also is considered to inhibit the function of HtrA1 [1] - [4].
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This part encodes for a type of inhibitor that is able to inhibit serine proteases and it is predicted to be located extracellularly. The main function of the inhibitor is to specifically target kallikrein-related peptide 5 (KLK-5) which is an important initiator of skin peeling. The inhibitor binds to trypsin proteases and since the catalytic core of HtrA1 (BBa_K4165004) is considered as a trypsin-like catalytic domain, so this inhibitor also is considered to inhibit the function of HtrA1 [1] - [4].
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===Functional Parameters===
 
===Functional Parameters===
 +
  
 
GC% Content
 
GC% Content
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58.9%
+
48.8%
  
 
Isoelectric point (PI)
 
Isoelectric point (PI)
−
7.362
+
8.834
  
 
Charge at pH 7
 
Charge at pH 7
−
0.609
+
6.831
  
 
Molecular Weight (Protein)
 
Molecular Weight (Protein)
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9.454 kDa
+
9.756 kDa
  
 +
===PDB Structure===
 
Only a predicted model (AlphaFold).
 
Only a predicted model (AlphaFold).
−
 
  
 
AlphaFold:
 
AlphaFold:
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https://alphafold.ebi.ac.uk/entry/O60575
+
https://alphafold.ebi.ac.uk/entry/Q5DT21
 
Molprobity:  
 
Molprobity:  
 
Clash Score:  
 
Clash Score:  

Revision as of 19:19, 5 October 2022


SPINK9 (Serine Peptidase Inhibitor Kazal type 9).

This basic part encodes Human serine protease inhibitor known as SPINK9 which is able to inhibit trypsin-like proteases, like HtrA1 (BBa_K4165004).


Usage and Biology

This part encodes for a type of inhibitor that is able to inhibit serine proteases and it is predicted to be located extracellularly. The main function of the inhibitor is to specifically target kallikrein-related peptide 5 (KLK-5) which is an important initiator of skin peeling. The inhibitor binds to trypsin proteases and since the catalytic core of HtrA1 (BBa_K4165004) is considered as a trypsin-like catalytic domain, so this inhibitor also is considered to inhibit the function of HtrA1 [1] - [4].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

GC% Content 48.8%

Isoelectric point (PI) 8.834

Charge at pH 7 6.831

Molecular Weight (Protein) 9.756 kDa

PDB Structure

Only a predicted model (AlphaFold).

AlphaFold: https://alphafold.ebi.ac.uk/entry/Q5DT21 Molprobity: Clash Score: Ramachandran Favoured: Ramachandran Outliers: Rotamers Outliers: C-beta Deviations: Q-Mean:

References

1 - Frochaux, V., Hildebrand, D., Talke, A., Linscheid, M. W., & Schlüter, H. (2014). Alpha-1-antitrypsin: a novel human high temperature requirement protease A1 (HTRA1) substrate in human placental tissue. PloS one, 9(10), e109483. 2 - Grau, S., Baldi, A., Bussani, R., Tian, X., Stefanescu, R., Przybylski, M., ... & Ehrmann, M. (2005). Implications of the serine protease HtrA1 in amyloid precursor protein processing. Proceedings of the National Academy of Sciences, 102(17), 6021-6026. 3 - Eigenbrot, C., Ultsch, M., Lipari, M. T., Moran, P., Lin, S. J., Ganesan, R., ... & Kirchhofer, D. (2012). Structural and functional analysis of HtrA1 and its subdomains. Structure, 20(6), 1040-1050. 4 - Chen, T. J., Tian, Y. F., Chou, C. L., Chan, T. C., He, H. L., Li, W. S., ... & Lai, H. Y. (2021). High spink4 expression predicts poor outcomes among rectal cancer patients receiving CCRT. Current Oncology, 28(4), 2373-2384.