Difference between revisions of "Part:BBa K4165157"
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Tau binding peptide targeting the PHF seed of Tau | Tau binding peptide targeting the PHF seed of Tau | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | MMDP2 peptide sequence is HSDLWRRSFELM, it binds to <sup>275</sup>-VQIINK-<sup>280</sup> (PHF*) and 306-VQIVYK-311 (PHF) motifs of tau that are reported to drive tau paired helical filament aggregates. it inhibits formation of PHF6* aggregates, but not PHF and full length tau driven aggregates. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4165157 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4165157 SequenceAndFeatures</partinfo> | ||
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+ | ===Dry Lab=== | ||
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+ | <p style=" font-weight: bold; font-size:14px;"> Modeling </p> | ||
+ | MMDP6 is modeled by AlphaFold2 and Apptest, best model obtained from AlphaFold2 | ||
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+ | <html> | ||
+ | <p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/mmdp6.png" style="margin-left:200px;" alt="" width="300" /></p> | ||
+ | </html> | ||
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+ | Figure 1.: Predicted 3D structure of Synthetic peptide MMDP6. | ||
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+ | <p style=" font-weight: bold; font-size:13px;"> Table 1: Quality assessment parameters of MMDP6 model. </p> | ||
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+ | <html> | ||
+ | <p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/mmdp6-qa.png" style="margin-left:75px;" alt="" width="800" /></p> | ||
+ | </html> | ||
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+ | ===References=== | ||
+ | 1. Malhis, M., Kaniyappan, S., Aillaud, I., Chandupatla, R. R., Ramirez, L. M., Zweckstetter, M., ... & Funke, S. A. (2021). Potent Tau Aggregation Inhibitor D‐Peptides Selected against Tau‐Repeat 2 Using Mirror Image Phage Display. ChemBioChem, 22(21), 3049-3059. | ||
Revision as of 19:05, 5 October 2022
MMDP6 Peptide
Tau binding peptide targeting the PHF seed of Tau
Usage and Biology
MMDP2 peptide sequence is HSDLWRRSFELM, it binds to 275-VQIINK-280 (PHF*) and 306-VQIVYK-311 (PHF) motifs of tau that are reported to drive tau paired helical filament aggregates. it inhibits formation of PHF6* aggregates, but not PHF and full length tau driven aggregates.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Dry Lab
Modeling
MMDP6 is modeled by AlphaFold2 and Apptest, best model obtained from AlphaFold2
Figure 1.: Predicted 3D structure of Synthetic peptide MMDP6.
Table 1: Quality assessment parameters of MMDP6 model.
References
1. Malhis, M., Kaniyappan, S., Aillaud, I., Chandupatla, R. R., Ramirez, L. M., Zweckstetter, M., ... & Funke, S. A. (2021). Potent Tau Aggregation Inhibitor D‐Peptides Selected against Tau‐Repeat 2 Using Mirror Image Phage Display. ChemBioChem, 22(21), 3049-3059.