Difference between revisions of "Part:BBa K4165088"

 
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<partinfo>BBa_K4165088 short</partinfo>
 
<partinfo>BBa_K4165088 short</partinfo>
  
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A serine protease inhibitor is used to inhibit the action of HTRA1
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This basic part encodes Human serine protease inhibitor WAP-four disulfide core domain 14 which is able to inhibit HtrA1 (BBa_K4165004).
  
  
  
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===Usage and Biology===
 
===Usage and Biology===
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This type of family encodes for a type of inhibitor that contains a motif which consists of 8 cysteine residues capable of forming four disulfide bonds at the core of the protease, thus inhibiting its action. The main function of this inhibitor is to prevent elastase-mediated tissue proteolysis. This type of inhibitor is very effective and has high affinity for trypsin-like proteases (serine proteases), and in our case it would act as an inhibitor for the trypsin-like catalytic domain of serine protease HtrA1[1]-[3].
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
 
===Functional Parameters===
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GC Content%
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67.5%
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Isoelectric point (PI)
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8.641
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Charge at pH 7
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6.373
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Molecular Weight (Protein)
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12.27 kDa
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===PDB structure===
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X-ray, NMR, and the predicted structures (AlphaFold2) are all present.
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X-ray
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https://www.rcsb.org/structure/1FLE
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Q_Mean =
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Ramachandran Favoured =
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Ramachandran Outliers =
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Clash Score =
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C-beta Deviation =
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Rotamers outliers =
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Total Score =
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NMR:
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https://www.rcsb.org/structure/2REL
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Q_Mean =
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Ramachandran Favoured =
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Ramachandran Outliers =
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Clash Score =
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C-beta Deviation =
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Rotamers outliers =
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Total Score =
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AlphaFold
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https://alphafold.ebi.ac.uk/entry/P19957
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Q_Mean =
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Ramachandran Favoured =
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Ramachandran Outliers =
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Clash Score =
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C-beta Deviation =
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Rotamers outliers =
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Total Score =
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===References===
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1. Clauss, A., Lilja, H., & Lundwall, Å. (2005). The evolution of a genetic locus encoding small serine proteinase inhibitors. Biochemical and biophysical research communications, 333(2), 383-389.
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2. Eigenbrot, C., Ultsch, M., Lipari, M. T., Moran, P., Lin, S. J., Ganesan, R., ... & Kirchhofer, D. (2012). Structural and functional analysis of HtrA1 and its subdomains. Structure, 20(6), 1040-1050.
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3. Grau, S., Baldi, A., Bussani, R., Tian, X., Stefanescu, R., Przybylski, M., ... & Ehrmann, M. (2005). Implications of the serine protease HtrA1 in amyloid precursor protein processing. Proceedings of the National Academy of Sciences, 102(17), 6021-6026.
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<partinfo>BBa_K4165088 parameters</partinfo>
 
<partinfo>BBa_K4165088 parameters</partinfo>
 
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Revision as of 18:39, 5 October 2022


WAP-four disulfide core domain 14 serine protease inhibitor.

This basic part encodes Human serine protease inhibitor WAP-four disulfide core domain 14 which is able to inhibit HtrA1 (BBa_K4165004).


Usage and Biology

This type of family encodes for a type of inhibitor that contains a motif which consists of 8 cysteine residues capable of forming four disulfide bonds at the core of the protease, thus inhibiting its action. The main function of this inhibitor is to prevent elastase-mediated tissue proteolysis. This type of inhibitor is very effective and has high affinity for trypsin-like proteases (serine proteases), and in our case it would act as an inhibitor for the trypsin-like catalytic domain of serine protease HtrA1[1]-[3].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 132
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 189
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

GC Content% 67.5%

Isoelectric point (PI) 8.641

Charge at pH 7 6.373

Molecular Weight (Protein) 12.27 kDa

PDB structure

X-ray, NMR, and the predicted structures (AlphaFold2) are all present.

X-ray https://www.rcsb.org/structure/1FLE Q_Mean = Ramachandran Favoured = Ramachandran Outliers = Clash Score = C-beta Deviation = Rotamers outliers = Total Score =

NMR: https://www.rcsb.org/structure/2REL Q_Mean = Ramachandran Favoured = Ramachandran Outliers = Clash Score = C-beta Deviation = Rotamers outliers = Total Score =

AlphaFold https://alphafold.ebi.ac.uk/entry/P19957 Q_Mean = Ramachandran Favoured = Ramachandran Outliers = Clash Score = C-beta Deviation = Rotamers outliers = Total Score =

References

1. Clauss, A., Lilja, H., & Lundwall, Å. (2005). The evolution of a genetic locus encoding small serine proteinase inhibitors. Biochemical and biophysical research communications, 333(2), 383-389. 2. Eigenbrot, C., Ultsch, M., Lipari, M. T., Moran, P., Lin, S. J., Ganesan, R., ... & Kirchhofer, D. (2012). Structural and functional analysis of HtrA1 and its subdomains. Structure, 20(6), 1040-1050. 3. Grau, S., Baldi, A., Bussani, R., Tian, X., Stefanescu, R., Przybylski, M., ... & Ehrmann, M. (2005). Implications of the serine protease HtrA1 in amyloid precursor protein processing. Proceedings of the National Academy of Sciences, 102(17), 6021-6026.