Difference between revisions of "Part:BBa K4388002:Design"

 
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Yan, Z.-B., Liang, J.-L., Niu, F.-X., Shen, Y.-P., & Liu, J.-Z. (2021). Enhanced Production of Pterostilbene in Escherichia coli Through Directed Evolution and Host Strain Engineering. Frontiers in Microbiology, 12. doi:10.1186/s12934-017-0644-6
 
Yan, Z.-B., Liang, J.-L., Niu, F.-X., Shen, Y.-P., & Liu, J.-Z. (2021). Enhanced Production of Pterostilbene in Escherichia coli Through Directed Evolution and Host Strain Engineering. Frontiers in Microbiology, 12. doi:10.1186/s12934-017-0644-6
  
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Mutations added to wild-type sequence from GenBank code: BBa_K1033001
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Mutations added to wild-type sequence from registry code: BBa_K1033001
  
 
===References===
 
===References===

Latest revision as of 18:19, 5 October 2022


Arabidopsis thaliana 4-Coumarate:CoA Ligase Mutant (At4CL L57I/L460H)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1223
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was codon-optimised for E.coli K12 strain, sequence had one PstI site which was removed to allow for RFC10 compatibility.


Source

Yan, Z.-B., Liang, J.-L., Niu, F.-X., Shen, Y.-P., & Liu, J.-Z. (2021). Enhanced Production of Pterostilbene in Escherichia coli Through Directed Evolution and Host Strain Engineering. Frontiers in Microbiology, 12. doi:10.1186/s12934-017-0644-6

Mutations added to wild-type sequence from registry code: BBa_K1033001

References