Difference between revisions of "Part:BBa K4321001"
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<partinfo>BBa_K4321001 SequenceAndFeatures</partinfo>
 
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===Reference===
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BFP sequence and map. SnapGene. (n.d.). Retrieved October 4, 2022, from https://www.snapgene.com/resources/plasmid-files/?set=fluorescent_protein_genes_and_plasmids&plasmid=BFP
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Education in microscopy and digital imaging. ZEISS Microscopy Online Campus | Jellyfish Fluorescent Proteins. (n.d.). Retrieved October 4, 2022, from https://zeiss-campus.magnet.fsu.edu/articles/probes/jellyfishfps.html
  
 
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Revision as of 17:52, 5 October 2022


BFP

This blue fluorescent protein (BFP) one of the 19 variants of Aequorea victoria derived green fluorescent protein (GFP).

Usage and Biology

This BFP differs from its predecessor in that it contains a tyrosine (Y) to histidine (H) substitution at position 66. This Y66H substitution results in the board excitation close to 380 namometers and emission at 448 namometers. This BFP variant carries an additional modification, the addition of a mammalian codon directly downstream of the start methionin allowing its expression in mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

BFP sequence and map. SnapGene. (n.d.). Retrieved October 4, 2022, from https://www.snapgene.com/resources/plasmid-files/?set=fluorescent_protein_genes_and_plasmids&plasmid=BFP

Education in microscopy and digital imaging. ZEISS Microscopy Online Campus | Jellyfish Fluorescent Proteins. (n.d.). Retrieved October 4, 2022, from https://zeiss-campus.magnet.fsu.edu/articles/probes/jellyfishfps.html