Difference between revisions of "Part:BBa K4204007:Design"
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===References=== | ===References=== | ||
+ | Yang J, Han YH, Im J, Seo SW. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol. 2021 Apr;17(4):421-427. doi: 10.1038/s41589-021-00736-3. Epub 2021 Feb 4. PMID: 33542534. |
Latest revision as of 17:22, 5 October 2022
Quality control system + LysPBC5
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 706
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 758
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1004
Design Notes
Applying the QC system seamlessly connected with PART or plasmid is best.
Source
Eukaryotic translation system.
References
Yang J, Han YH, Im J, Seo SW. Synthetic protein quality control to enhance full-length translation in bacteria. Nat Chem Biol. 2021 Apr;17(4):421-427. doi: 10.1038/s41589-021-00736-3. Epub 2021 Feb 4. PMID: 33542534.