Difference between revisions of "Part:BBa K4165076"

Revision as of 16:44, 5 October 2022

SPINK1 (Serine Peptidase Inhibitor Kazal type 1).

This basic part encodes Human serine protease inhibitor known as SPINK1 which is able to inhibit trypsin-like proteases, like HtrA1 (BBa_K4165004).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4165076 short</partinfo>
-A serine protease inhibitor that is used to inhibit the proteolytic action of HTRA1
+This basic part encodes Human serine protease inhibitor known as SPINK1 which is able to inhibit trypsin-like proteases, like HtrA1 (BBa_K4165004).
 <!-- Add more about the biology of this part here
-===Usage and Biology===
+This type of family encodes for a type of inhibitor that is able to inhibit trypsins. The inhibitor is secreted from pancreatic acinar cells into the pancreatic juice. The main function of the inhibitor in pancreatic juice is the prevention of activation of immature trypsin zymogens within the pancreatic duct. The inhibitor binds to trypsin proteases and since the catalytic core of HtrA1 (BBa_K4165004) is considered as a trypsin-like catalytic domain, so this inhibitor also is considered to inhibit the function of HtrA1 [1] - [3].
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 <!-- Uncomment this to enable Functional Parameter display
-===Functional Parameters===
+GC% Content
+.5%
+Isoelectric point (PI)
+.338
+Charge at pH 7
+.508
+Molecular Weight (Protein)
+.507 kDa
+It has both x-ray structure and a predicted model (AlphaFold2).
+PDB structure
+X-ray structure: https://drive.google.com/drive/folders/1o_e_vCFs-bwbqS0z-3XsDzY9ENKnag4I
+Molprobity: 2.47
+Clash Score: 9.35
+Ramachandran Favoured: 94.44%
+Ramachandran Outliers: 0%
+Rotamers Outliers: 6%
+C-beta Deviations: 0%
+Q-Mean: 0.83 土 0.11
+AlphaFold:
+https://drive.google.com/drive/folders/1o_e_vCFs-bwbqS0z-3XsDzY9ENKnag4I
+Molprobity: 1.11
+Clash Score: 0
+Ramachandran Favoured: 92.21%
+Ramachandran Outliers: 0%
+Rotamers Outliers: 1.49%
+C-beta Deviations: 0%
+Q-Mean: 0.65 土 0.1
+References:
+- Frochaux, V., Hildebrand, D., Talke, A., Linscheid, M. W., & Schlüter, H. (2014). Alpha-1-antitrypsin: a novel human high temperature requirement protease A1 (HTRA1) substrate in human placental tissue. PloS one, 9(10), e109483.
+- Grau, S., Baldi, A., Bussani, R., Tian, X., Stefanescu, R., Przybylski, M., ... & Ehrmann, M. (2005). Implications of the serine protease HtrA1 in amyloid precursor protein processing. Proceedings of the National Academy of Sciences, 102(17), 6021-6026.
+- Eigenbrot, C., Ultsch, M., Lipari, M. T., Moran, P., Lin, S. J., Ganesan, R., ... & Kirchhofer, D. (2012). Structural and functional analysis of HtrA1 and its subdomains. Structure, 20(6), 1040-1050.
 <partinfo>BBa_K4165076 parameters</partinfo>
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