Difference between revisions of "Part:BBa K257006:Design"

(→‎Design Notes)
Line 7: Line 7:
 
===Design Notes===
 
===Design Notes===
  
−
ompA signal peptide for the secretion vectors
+
Amplified from the secretion vector pIN-III-ompA-2 (Ghrayeb et al, 1984) keeping the RBS
  
−
(i)functions efficiently to secrete a large amount of the OmpA protein
+
# functions efficiently to secrete a large amount of the OmpA protein
 +
# does not require any modification of the cleavage site during the secretion process, in contrast to the polipoprotein
  
−
(ii) does not require any modification of the cleavage site during the secretion process, in contrast to the polipoprotein. (ref Ghrayeb et al 1984)
+
C-term fusion using RFC23 (siver)
  
 
===Source===
 
===Source===
  
−
genomic DNA (K12)
+
pIN-III-ompA-2
  
 
===References===
 
===References===
 +
Ghrayeb et al 1984 - "Secretion Cloning Vectors in Escherichia coli" The EMBO Journal 3(10):2437-2442

Revision as of 09:28, 10 September 2009

ompA sequence signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amplified from the secretion vector pIN-III-ompA-2 (Ghrayeb et al, 1984) keeping the RBS

  1. functions efficiently to secrete a large amount of the OmpA protein
  2. does not require any modification of the cleavage site during the secretion process, in contrast to the polipoprotein

C-term fusion using RFC23 (siver)

Source

pIN-III-ompA-2

References

Ghrayeb et al 1984 - "Secretion Cloning Vectors in Escherichia coli" The EMBO Journal 3(10):2437-2442