Difference between revisions of "Part:BBa K259006"
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==GFP Structure== | ==GFP Structure== | ||
[[Image:BCCS_600px-GFP_structure.png|center|500px|frame|The Structure of GFP. Image courtesy of http://www.wwpdb.org/ PDB]] | [[Image:BCCS_600px-GFP_structure.png|center|500px|frame|The Structure of GFP. Image courtesy of http://www.wwpdb.org/ PDB]] | ||
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| + | ==Terminator Structure== | ||
| + | [[Image:Mfold-B0014-1.png|center|500px|frame|The structure/sequence of the double terminator. Image from the original [https://parts.igem.org/Part:BBa_B0014 part page ]]] | ||
==Part Description== | ==Part Description== | ||
Revision as of 18:57, 9 September 2009
GFP-Terminator
GFP Structure
The Structure of GFP. Image courtesy of http://www.wwpdb.org/ PDB
Terminator Structure
The structure/sequence of the double terminator. Image from the original part page
Part Description
This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator.
Green Fluorescent protein (originally from part BBa_E0040)will glow green when excited by the correct wavelengths and thus can be used as a reporter gene. This part is directly fused to a terminator (originally from part BBa_B0014) so it is ideal to be fused at the end of your construct.
Table of Statistics
| Chassis | Monomer/Multimer | Aminoacid Length/Weight | Localisation | Structure | Excitation wavelengths | Emmision wavelengths |
|---|---|---|---|---|---|---|
| Prokaryotic and Eukaryotic | Bipartite | 274 aminoacids | beta-barrel(for GFP) hairpins for Terminator | 501nm | 511nm |
References
<biblio>
- Valdivia pmid=8707053
</biblio>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644