Difference between revisions of "Part:BBa K259006"

Revision as of 18:42, 9 September 2009

GFP-Terminator

Part Description

This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator.

Green Fluorescent protein (originally from part BBa_E0040)will glow green when excited by the correct wavelengths and thus can be used as a reporter gene. This part is directly fused to a terminator (originally from part BBa_B0014) so it is ideal to be fused at the end of your construct.

Table of Statistics

Chassis	Monomer/Multimer	Aminoacid Length/Weight	Localisation	Structure	Excitation wavelengths	Emmision wavelengths
Prokaryotic and Eukaryotic	Bipartite	274 aminoacids		beta-barrel(for GFP) hairpins for Terminator	501nm	511nm

References

Valdivia pmid=8707053

</biblio>

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 644

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K259006 short</partinfo>
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 This is a composite part made from Green Fluorescent Protein (GFP) and a double terminator.
-Green Fluorescent protein (originally from part [https://parts.igem.org/Part:BBa_E0040 BBa_E0040]) that will glow green when excited by the correct wavelengths and thus can be used as a reporter gene.
+Green Fluorescent protein (originally from part [https://parts.igem.org/Part:BBa_E0040 BBa_E0040])will glow green when excited by the correct wavelengths and thus can be used as a reporter gene.
 This part is directly fused to a terminator (originally from part [https://parts.igem.org/Part:BBa_B0014 BBa_B0014]) so it is ideal to be fused at the end of your construct.