Difference between revisions of "Part:BBa K4137001"
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<partinfo>BBa_K4137001 short</partinfo> | <partinfo>BBa_K4137001 short</partinfo> | ||
− | + | BBa_K4137001 codes for the CcdB protein, a toxin to many bacteria including E. coli and B. subtilis. Through the formation of | |
− | + | a CcdA-CcdB complex by binding with CcdA, CcdB loses its ability to interact with DNA gyrase thus losing its toxicity. A Myc tag is added for protein purification, identification, and distinguishment from CcdA. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 06:44, 5 October 2022
CcdB-Myc Toxin
BBa_K4137001 codes for the CcdB protein, a toxin to many bacteria including E. coli and B. subtilis. Through the formation of
a CcdA-CcdB complex by binding with CcdA, CcdB loses its ability to interact with DNA gyrase thus losing its toxicity. A Myc tag is added for protein purification, identification, and distinguishment from CcdA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 216