Difference between revisions of "Part:BBa K4463003"

 
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This is a composite part that consist of CsgA and MBP3. CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of MBP3, which can bind Ag(I) and produce nano Ag, achieved by linking to csgA. MBP3 is identified from a combinatorial phage display peptide library, for more information about MBP3, please visit the page of <a href="https://parts.igem.org/Part:BBa_K4463000">MBP3</a> that created by IGEM XJTLU_China 2023.
  
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CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of MBP3, which can bind Ag(I) and produce nano Ag, achieved by linking to csgA.
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We have tested the function of CsgA-MBP3 part and compared with Mg1655 as control.
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<img src="https://static.igem.wiki/teams/4463/wiki/figures-for-engineering-and-parts/mbp3.png
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Figure1. In different concentrations of AgNO3 solution, modified <i>E.coli</i> that express MBP3 adsorbed more Ag(I) than Mg1655 pGEX-4T-1, which is the control group.
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We used ICP-MS to measure the unbinding Ag(I) in the supernatant, for detailed information about our measurement method, please visit our method on wiki.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:40, 5 October 2022


csgA-MBP3
This is a composite part that consist of CsgA and MBP3. CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of MBP3, which can bind Ag(I) and produce nano Ag, achieved by linking to csgA. MBP3 is identified from a combinatorial phage display peptide library, for more information about MBP3, please visit the page of MBP3 that created by IGEM XJTLU_China 2023. We have tested the function of CsgA-MBP3 part and compared with Mg1655 as control.

Figure1. In different concentrations of AgNO3 solution, modified E.coli that express MBP3 adsorbed more Ag(I) than Mg1655 pGEX-4T-1, which is the control group.

We used ICP-MS to measure the unbinding Ag(I) in the supernatant, for detailed information about our measurement method, please visit our method on wiki.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]