Difference between revisions of "Part:BBa K4167000"
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To construct the standard part, toehold switch-amilCP was synthesized and checked the restriction enzyme information, which is showed as follows: | To construct the standard part, toehold switch-amilCP was synthesized and checked the restriction enzyme information, which is showed as follows: | ||
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| + | [[File:K4167000-fig.1.jpg|center]] | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 03:06, 5 October 2022
toehold switch-amilCP
Toehold switch-amilCP was inserted into pSB1C3 which is designed to express amilCP protein triggered by miRNA 34a-5p. It is used to detect the amount of miRNA 34a-5p in samples.
To construct the standard part, toehold switch-amilCP was synthesized and checked the restriction enzyme information, which is showed as follows:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]