Difference between revisions of "Part:BBa K4167000"
Line 4: Line 4:
  
 
Toehold switch-amilCP was inserted into pSB1C3 which is designed to express amilCP protein triggered by miRNA 34a-5p. It is used to detect the amount of miRNA 34a-5p in samples.  
 
Toehold switch-amilCP was inserted into pSB1C3 which is designed to express amilCP protein triggered by miRNA 34a-5p. It is used to detect the amount of miRNA 34a-5p in samples.  
 +
<br/>
 +
<br/>
 +
To construct the standard part, toehold switch-amilCP was synthesized and checked the restriction enzyme information, which is showed as follows:
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:03, 5 October 2022


toehold switch-amilCP

Toehold switch-amilCP was inserted into pSB1C3 which is designed to express amilCP protein triggered by miRNA 34a-5p. It is used to detect the amount of miRNA 34a-5p in samples.

To construct the standard part, toehold switch-amilCP was synthesized and checked the restriction enzyme information, which is showed as follows:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]