Difference between revisions of "Part:BBa K4323000"
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Secreted, cytolytic toxin that forms pores in host membranes after proteolytic removal of a C-terminal propeptide, leading to destruction of the membrane permeability barrier and host cell death. The pores are formed by transmembrane beta-strands and are approximately 3 nm in diameter. This part has been codon optimized for expression in Escherichia coli. | Secreted, cytolytic toxin that forms pores in host membranes after proteolytic removal of a C-terminal propeptide, leading to destruction of the membrane permeability barrier and host cell death. The pores are formed by transmembrane beta-strands and are approximately 3 nm in diameter. This part has been codon optimized for expression in Escherichia coli. | ||
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| + | [[File:Aerolysin toxicity.png|800px|center|FitD toxicity]] | ||
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Revision as of 23:12, 4 October 2022
Coding sequence for Aerolysin
Secreted, cytolytic toxin that forms pores in host membranes after proteolytic removal of a C-terminal propeptide, leading to destruction of the membrane permeability barrier and host cell death. The pores are formed by transmembrane beta-strands and are approximately 3 nm in diameter. This part has been codon optimized for expression in Escherichia coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 206
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 75
Illegal AgeI site found at 313
Illegal AgeI site found at 1270 - 1000COMPATIBLE WITH RFC[1000]