Difference between revisions of "Part:BBa K4323002"

Line 5: Line 5:
 
This part is a truncated version of FitD, a molluscicide from <em>Pseudomonas protegens</em>. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to BBa_K2683006.
 
This part is a truncated version of FitD, a molluscicide from <em>Pseudomonas protegens</em>. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to BBa_K2683006.
  
[[File:FitD toxicity.png|400px|center|FitD toxicity]]
+
[[File:FitD toxicity.png|800px|center|FitD toxicity]]
  
  

Revision as of 22:53, 4 October 2022


Truncated FitD

This part is a truncated version of FitD, a molluscicide from Pseudomonas protegens. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to BBa_K2683006.

FitD toxicity




Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 909
    Illegal BamHI site found at 1019
    Illegal BamHI site found at 2568
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2191
  • 1000
    COMPATIBLE WITH RFC[1000]