Difference between revisions of "Part:BBa K4174002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Based on the 2006 MIT iGEM team's part BBa_J45995, with GFP replaced with sfGFP from Ceroni <i>et al.</i> 2015, RBS BBa_B0030 replaced with an RBS containing region from Ceroni <i>et al.</i> 2015, and scar sequences removed. | + | Based on the 2006 MIT iGEM team's composite part BBa_J45995, with GFP replaced with sfGFP from Ceroni <i>et al.</i> 2015, RBS BBa_B0030 replaced with an RBS containing region from Ceroni <i>et al.</i> 2015, and scar sequences removed. |
===Source=== | ===Source=== | ||
Ceroni, F., Algar, R., Stan, G., & Ellis, T. (2015). Quantifying cellular capacity identifies gene expression designs with reduced burden. <i>Nature Methods,</i> 12(5):415-418. Doi: 10.1038/nmeth.3339 | Ceroni, F., Algar, R., Stan, G., & Ellis, T. (2015). Quantifying cellular capacity identifies gene expression designs with reduced burden. <i>Nature Methods,</i> 12(5):415-418. Doi: 10.1038/nmeth.3339 | ||
Revision as of 22:29, 4 October 2022
osmY-sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 419
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Based on the 2006 MIT iGEM team's composite part BBa_J45995, with GFP replaced with sfGFP from Ceroni et al. 2015, RBS BBa_B0030 replaced with an RBS containing region from Ceroni et al. 2015, and scar sequences removed.
Source
Ceroni, F., Algar, R., Stan, G., & Ellis, T. (2015). Quantifying cellular capacity identifies gene expression designs with reduced burden. Nature Methods, 12(5):415-418. Doi: 10.1038/nmeth.3339