Difference between revisions of "Part:BBa K4368005"
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It is a composition based on the ''cI'' repressor coding region of bacteriophage lambda, which features an LVA tail for rapid degradation of the protein (<partinfo>BBa_C0051</partinfo>). In the case of our team, it has been placed under the control of the pcstA promoter (<partinfo>BBa_K118011</partinfo>), together with a strong rbs (<partinfo>BBa_B0030</partinfo>) and double terminator (<partinfo>BBa_B0015</partinfo>). This part of our group's collection, iGEM22_UMA_MALAGA, is used as a mechanism of gene regulation, as the transcription and subsequent translation of the repressor protein will be influenced by the concentration of glucose generated in the medium by the transformed bacteria.  
 
It is a composition based on the ''cI'' repressor coding region of bacteriophage lambda, which features an LVA tail for rapid degradation of the protein (<partinfo>BBa_C0051</partinfo>). In the case of our team, it has been placed under the control of the pcstA promoter (<partinfo>BBa_K118011</partinfo>), together with a strong rbs (<partinfo>BBa_B0030</partinfo>) and double terminator (<partinfo>BBa_B0015</partinfo>). This part of our group's collection, iGEM22_UMA_MALAGA, is used as a mechanism of gene regulation, as the transcription and subsequent translation of the repressor protein will be influenced by the concentration of glucose generated in the medium by the transformed bacteria.  
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In the case of high glucose concentration, the ''pcstA'' promoter is repressed, preventing the synthesis of the cellulolytic enzymes (controlled under the same promoter), ''GFP'' (<partinfo>BBa_K4368006</partinfo>) and the ''cI'' protein (<partinfo>BBa_K4368005</partinfo>). In contrast, the ''glgC16''-based construct (<partinfo>BBa_K4368004</partinfo>) would be activated. In contrast, when the glucose concentration is low, the ''pcstA'' promoter can transcribe the genes coding for cellulolytic enzymes (<partinfo>BBa_K4368000</partinfo>, <partinfo>BBa_K4368001</partinfo> and <partinfo>BBa_K4368003</partinfo>) and ''RFP'' (<partinfo>BBa_K4368007</partinfo>).
 
  
  

Revision as of 22:22, 4 October 2022


pcstA + rbs + cI + terminator

It is a composition based on the cI repressor coding region of bacteriophage lambda, which features an LVA tail for rapid degradation of the protein (BBa_C0051). In the case of our team, it has been placed under the control of the pcstA promoter (BBa_K118011), together with a strong rbs (BBa_B0030) and double terminator (BBa_B0015). This part of our group's collection, iGEM22_UMA_MALAGA, is used as a mechanism of gene regulation, as the transcription and subsequent translation of the repressor protein will be influenced by the concentration of glucose generated in the medium by the transformed bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]