Difference between revisions of "Part:BBa K4368005"

Revision as of 22:15, 4 October 2022

pcstA + rbs + cI + terminator It is a composition based on the cI repressor coding region of bacteriophage lambda, which features an LVA tail for rapid degradation of the protein (BBa_C0051). In the case of our team, it has been placed under the control of the pcstA promoter (BBa_K118011), together with a strong rbs (BBa_B0030) and double terminator (BBa_B0015). This part of our group's collection, iGEM22_UMA_MALAGA, is used as a mechanism of gene regulation, as the transcription and subsequent translation of the repressor protein will be influenced by the concentration of glucose generated in the medium by the transformed bacteria. In the case of high glucose concentration, the pcstA promoter is repressed, preventing the synthesis of the cellulolytic enzymes (controlled under the same promoter), GFP (BBa_K4368006) and the cI protein (BBa_K4368005). In contrast, the glgC16-based construct (BBa_K4368004) would be activated. In contrast, when the glucose concentration is low, the pcstA promoter can transcribe the genes coding for cellulolytic enzymes (BBa_K4368000, BBa_K4368001 and BBa_K4368003) and RFP (BBa_K4368007).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4368005 short</partinfo>
+It is a composition based on the ''cI'' repressor coding region of bacteriophage lambda, which features an LVA tail for rapid degradation of the protein (<partinfo>BBa_C0051</partinfo>). In the case of our team, it has been placed under the control of the pcstA promoter (BBa_K118011), together with a strong rbs (<partinfo>BBa_B0030</partinfo>) and double terminator (<partinfo>BBa_B0015</partinfo>). This part of our group's collection, iGEM22_UMA_MALAGA, is used as a mechanism of gene regulation, as the transcription and subsequent translation of the repressor protein will be influenced by the concentration of glucose generated in the medium by the transformed bacteria.
+In the case of high glucose concentration, the ''pcstA'' promoter is repressed, preventing the synthesis of the cellulolytic enzymes (controlled under the same promoter), GFP (<partinfo>BBa_K4368006</partinfo>) and the ''cI'' protein (<partinfo>BBa_K4368005</partinfo>). In contrast, the ''glgC16''-based construct (<partinfo>BBa_K4368004</partinfo>) would be activated. In contrast, when the glucose concentration is low, the ''pcstA'' promoter can transcribe the genes coding for cellulolytic enzymes (<partinfo>BBa_K4368000</partinfo>, <partinfo>BBa_K4368001</partinfo> and <partinfo>BBa_K4368003</partinfo>) and ''RFP'' (<partinfo>BBa_K4368007</partinfo>).
-xxx
 <!-- Add more about the biology of this part here