Difference between revisions of "Part:BBa J428351"

(Cambridge 2022 DV Characterisation)
Line 5: Line 5:
 
== Characterisation Studies ==
 
== Characterisation Studies ==
  
===Cambridge 2022 DV Characterisation ===
+
===Cambridge 2022 JUMP DV Characterisation ===
 
<ul>
 
<ul>
 
   <li>Summary</li>
 
   <li>Summary</li>
Line 17: Line 17:
  
 
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 +
 
==DNA Sequence and Features==
 
==DNA Sequence and Features==
 
<partinfo>BBa_J428351 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_J428351 SequenceAndFeatures</partinfo>

Revision as of 17:33, 4 October 2022

pJUMP27-1A KanR Type IIS Level 1 vector. Origin pSC101 (low copy number)


Characterisation Studies

Cambridge 2022 JUMP DV Characterisation

  • Summary
  • Verdict
  • Experience/Detailed Protocol: Link here

DNA Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 3023
    Illegal suffix found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3023
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NotI site found at 24
    Illegal NotI site found at 3029
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3023
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3023
    Illegal suffix found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3023
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3038
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NgoMIV site found at 1390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.