Difference between revisions of "Part:BBa J428351"
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===Sequence and Features===
 
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Revision as of 17:30, 4 October 2022


pJUMP27-1A KanR Type IIS Level 1 vector. Origin pSC101 (low copy number)

pJUMP27-1A KanR Type IIS Level 1 vector. Origin pSC101 (low copy number)


Characterisation Studies

Cambridge 2022 DV Characterisation

  • Summary
  • Verdict
  • Experience/Detailed Protocol: Link here

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 3023
    Illegal suffix found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3023
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NotI site found at 24
    Illegal NotI site found at 3029
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3023
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3023
    Illegal suffix found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3023
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3038
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NgoMIV site found at 1390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.