Difference between revisions of "Part:BBa K4368004"

Revision as of 17:28, 4 October 2022

pcI + rbs + glgC16 + terminator

GlgC16 encodes for the ADP-glucose pyrophosphorylase gene of Escherichia coli BBa_B0010(BBa_K118016). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by the cI protein of the phage lambda (BBa_R1051). The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 272
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4368004 short</partinfo>
-''GlgC16'' encodes for the ADP-glucose pyrophosphorylase gene of ''Escherichia coli'' (<bbpart>BBa_B0010</bbpart>[[BBa_K118016]]). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0030 BBa_B0030]), a double terminator ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]) as well as a promoter inducible by the cI protein of the ''phage lambda'' ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R1051 BBa_R1051]).
+''GlgC16'' encodes for the ADP-glucose pyrophosphorylase gene of ''Escherichia coli'' <bbpart>BBa_B0010</bbpart>([[BBa_K118016]]). This enzyme is responsible for the synthesis of starch-like starch using glucose as a substrate. In addition, this part includes the composition used by the team, which includes a strong rbs ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0030 BBa_B0030]), a double terminator ([https://parts.igem.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]) as well as a promoter inducible by the cI protein of the ''phage lambda'' ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R1051 BBa_R1051]).
 The gene has been placed under the control of this promoter to build the glucose concentration-based gene regulatory circuit that integrates all our parts.