Difference between revisions of "Part:BBa K4115014"

Revision as of 14:27, 4 October 2022

PnifH（with RBS）

The promoter of the nifH coding sequence on the nitrogen-fixing gene cluster is regulated by the nifA protein.

Usage and Biology

The nitrogen-fixing gene cluster comes into play due to the downstream protein expression of PnifH in the original A. caul genome[1]. The PnifH fragment was added to our designed system and sfGFP(BBa_K4115000) was expressed downstream to verify whether the artificially designed system could work normally in A. caul.
[1]Ryu, MH., Zhang, J., Toth, T. et al. Control of nitrogen fixation in bacteria that associate with cereals. Nat Microbiol 5, 314–330 (2020). https://doi.org/10.1038/s41564-019-0631-2 Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 272

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-The nitrogen-fixing gene cluster comes into play due to the downstream protein expression of PnifH in the A. caul progenome[1]. The PnifH fragment was added to our designed system and sfGFP(<partinfo>BBa_K4115000</partinfo>) was expressed downstream to verify whether the artificially designed system could work normally in A. caul.<br>
+The nitrogen-fixing gene cluster comes into play due to the downstream protein expression of PnifH in the original A. caul genome[1]. The PnifH fragment was added to our designed system and sfGFP(<partinfo>BBa_K4115000</partinfo>) was expressed downstream to verify whether the artificially designed system could work normally in A. caul.<br>
 [1]Ryu, MH., Zhang, J., Toth, T. et al. Control of nitrogen fixation in bacteria that associate with cereals. Nat Microbiol 5, 314–330 (2020). https://doi.org/10.1038/s41564-019-0631-2
 <span class='h3bb'>Sequence and Features</span>