Difference between revisions of "Part:BBa K4212049:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
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x
+
PsspB is a promoter, which can aid the expression of gerA. GerA is one of the germinant receptors of B.subtilis, and it can respond to the presence of L-alanine and increase the germinantion rate of B.subtilis. The previous iGEM team iGEM17_SZU-China proved the superior transcription efficiency of this promoter.
  
  
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===Source===
 
===Source===
  
−
x
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Synthetic construct.
  
 
===References===
 
===References===

Revision as of 13:56, 4 October 2022


Ger1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3591
    Illegal PstI site found at 606
    Illegal PstI site found at 3542
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3591
    Illegal PstI site found at 606
    Illegal PstI site found at 3542
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3591
    Illegal BglII site found at 3123
    Illegal XhoI site found at 713
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3591
    Illegal PstI site found at 606
    Illegal PstI site found at 3542
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3591
    Illegal PstI site found at 606
    Illegal PstI site found at 3542
    Illegal NgoMIV site found at 66
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2778


Design Notes

PsspB is a promoter, which can aid the expression of gerA. GerA is one of the germinant receptors of B.subtilis, and it can respond to the presence of L-alanine and increase the germinantion rate of B.subtilis. The previous iGEM team iGEM17_SZU-China proved the superior transcription efficiency of this promoter.


Source

Synthetic construct.

References