Difference between revisions of "Part:BBa K2116002:Experience"

 
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===Applications of BBa_K2116002===
 
===Applications of BBa_K2116002===
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This composite part consists of the ETH promoter NorV (<html><a href="https://parts.igem.org/Part:BBa_K2116002">BBa_K2116002</a></html>), superfolder GFP preceded by one strong ribosomal binding site (<html><a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a></html>, <html><a href="https://parts.igem.org/Part:BBa_K2553008">BBa_K2553008</a></html>), the NorR regulator (<html><a href="https://parts.igem.org/Part:BBa_K4387001">BBa_K4387001</a></html>), and a double forward (<html><a href="https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a></html>). We chose a high-copy backbone from Twist for this part. We wanted to compare this ETH NorV promoter to the pNorVβ promoter and see which one was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team when tested at different concentration levels.
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This construct was tested in the bacterial strain E.coli Nissle 1917.
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=Characterization=
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===Measurements===
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[[File:DoseResponseETH.jpeg|450px|thumb|left|'''Figure 1:''' Induction response of the 2016 ETH Team NorV promoter to different DETA/NO concentrations with respect to time.]]
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[[File:ResponseETH.jpeg|450px|thumb|left|'''Figure 2:''' Dose response of the 2016 ETH Team NorV promoter at different DETA/NO concentrations.]]
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[[File:DoseResponse1rbs.jpeg|450px|thumb|left|'''Figure 1:''' Induction response of the pNorVβ promoter to different DETA/NO concentrations with respect to time.]]
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[[File:Response1rbs.jpeg|450px|thumb|left|'''Figure 2:''' Dose response of the pNorVβ promoter at different DETA/NO concentrations.]]
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===References===
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Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223
  
 
===User Reviews===
 
===User Reviews===

Revision as of 13:46, 4 October 2022


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Please enter how you used this part and how it worked out.

Applications of BBa_K2116002

This composite part consists of the ETH promoter NorV (BBa_K2116002), superfolder GFP preceded by one strong ribosomal binding site (BBa_B0034, BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward (BBa_B0015). We chose a high-copy backbone from Twist for this part. We wanted to compare this ETH NorV promoter to the pNorVβ promoter and see which one was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team when tested at different concentration levels.

This construct was tested in the bacterial strain E.coli Nissle 1917.


Characterization

Measurements

Figure 1: Induction response of the 2016 ETH Team NorV promoter to different DETA/NO concentrations with respect to time.
Figure 2: Dose response of the 2016 ETH Team NorV promoter at different DETA/NO concentrations.
Figure 1: Induction response of the pNorVβ promoter to different DETA/NO concentrations with respect to time.
Figure 2: Dose response of the pNorVβ promoter at different DETA/NO concentrations.


References

Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223

User Reviews

UNIQ3d91b51663c6d0b1-partinfo-00000005-QINU UNIQ3d91b51663c6d0b1-partinfo-00000006-QINU