Difference between revisions of "Part:BBa K4387004"
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[[File:ResponseETH.jpeg|450px|thumb|left|'''Figure 2:''' Dose response of the 2016 ETH Team NorV promoter at different DETA/NO concentrations.]] | [[File:ResponseETH.jpeg|450px|thumb|left|'''Figure 2:''' Dose response of the 2016 ETH Team NorV promoter at different DETA/NO concentrations.]] | ||
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Revision as of 13:44, 4 October 2022
Nitric Oxide Sensing Genetic Circuit with Promoter BBa_K2116002
Usage and Biology
This composite part consists of the ETH promoter NorV (BBa_K2116002), superfolder GFP preceded by one strong ribosomal binding site (BBa_B0034, BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward (BBa_B0015). We chose a high-copy backbone from Twist for this part. We wanted to compare this ETH NorV promoter to the pNorVβ promoter and see which one was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team when tested at different concentration levels.
This construct was tested in the bacterial strain E.coli Nissle 1917.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 742
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
Measurements