Difference between revisions of "Part:BBa K4229021"

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===Usage and Biology===

===Usage and Biology===

Short Description:  

Short Description:  

Besides the well-known 21 canonical amino acids, there is a variety of non-canonical amino acids which can be used to further modify proteins. One way of incorporating non-canonical amino acids is via the amber stop codon suppression technology. This part, sfGFP G4, is a model protein to test non-canonical amino acid incorporation. Without successful incorporation, a non-functional version of sfGFP will be translated, and therefore not give a fluorescence signal. However, after successful incorporation of the non-canonical amino acid, fluorescence will be restored providing an immediate readout of the efficiency of amber stop codon suppression via orthogonal translation systems.  

Besides the well-known 21 canonical amino acids, there is a variety of non-canonical amino acids which can be used to further modify proteins. One way of incorporating non-canonical amino acids is via the amber stop codon suppression technology. This part, sfGFP G4, is a model protein to test non-canonical amino acid incorporation. Without successful incorporation, a non-functional version of sfGFP will be translated, and therefore not give a fluorescence signal. However, after successful incorporation of the non-canonical amino acid, fluorescence will be restored providing an immediate readout of the efficiency of amber stop codon suppression via orthogonal translation systems.  

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