Difference between revisions of "Part:BBa K4387004"

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===Usage and Biology===
 
===Usage and Biology===
  
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This composite part consists of the ETH promoter NorV (<html><a href="http://https://parts.igem.org/Part:BBa_K2116002">BBa_K2116002</a></html>), a superfolder GFP (<html><a href="http://https://parts.igem.org/Part:BBa_K2553008">BBa_K2553008</a></html>), the NorR regulator (<html><a href="http://https://parts.igem.org/Part:BBa_K4387001">BBa_K4387001</a></html>), and a double forward terminator (<html><a href="http://https://parts.igem.org/Part:BBa_B0015">BBa_B0015</a></html>). We wanted to test which promoter was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team.
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This composite part consists of the ETH promoter NorV (<html><a href="https://parts.igem.org/Part:BBa_K2116002">BBa_K2116002</a></html>), a superfolder GFP [https://parts.igem.org/Part:BBa_K2553008 (BBa_K2553008),]the NorR regulator [https://parts.igem.org/Part:BBa_K4387001 (BBa_K4387001),]and a double forward terminator[https://parts.igem.org/Part:BBa_B0015 (BBa_B0015).] We wanted to test which promoter was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team.
  
  

Revision as of 11:10, 4 October 2022


Nitric Oxide Sensing Genetic Circuit with Promoter BBa_K2116002

Usage and Biology

This composite part consists of the ETH promoter NorV (BBa_K2116002), a superfolder GFP (BBa_K2553008),the NorR regulator (BBa_K4387001),and a double forward terminator(BBa_B0015). We wanted to test which promoter was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team.


This construct was tested in the bacterial strain E.coli Nissle 1917.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 742
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Measurements

Figure 1: Induction response of the 2016 ETH Team NorV promoter to different DETA/NO concentrations with respect to time.

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Figure 2: Dose response of the 2016 ETH Team NorV promoter at different DETA/NO concentrations.