Difference between revisions of "Part:BBa K4463004"

 
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CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA, a protein belongs to Metallothionein family, achieved by linking to csgA.
 
CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA, a protein belongs to Metallothionein family, achieved by linking to csgA.
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IGEM_XJTLU 2023 verified the function of CsgA-SmtA by measuring the concentration of Cu(II) and Cd(II) adsorbed by SmtA protein on the surface of <i>E.coli</i>.
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<img src="https://static.igem.wiki/teams/4463/wiki/figures-for-engineering-and-parts/smta-adsorb-cu-1.png
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Figure1. <i>E.coli</i> expressed SmtA protein showed higher adsorption of Cu(II) in 1.8mM CuSO4 solution.
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<img src="https://static.igem.wiki/teams/4463/wiki/figures-for-engineering-and-parts/smta-adsorb-cd1.png
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Figure1. <i>E.coli</i> expressed SmtA protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:58, 4 October 2022


CsgA-SmtA
CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA, a protein belongs to Metallothionein family, achieved by linking to csgA.

IGEM_XJTLU 2023 verified the function of CsgA-SmtA by measuring the concentration of Cu(II) and Cd(II) adsorbed by SmtA protein on the surface of E.coli.

Figure1. E.coli expressed SmtA protein showed higher adsorption of Cu(II) in 1.8mM CuSO4 solution.

Figure1. E.coli expressed SmtA protein showed higher adsorption of Cd(II) in 300μM CdCl2 solution.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 561
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 561
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 561
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 561
    Illegal AgeI site found at 589
  • 1000
    COMPATIBLE WITH RFC[1000]