Difference between revisions of "Part:BBa K4115041"

Revision as of 08:13, 4 October 2022

nifA_upstream-BleoR-nifA_downstream Add the upstream and downstream fragments of the nitrogen-fixing bacteria (A. caul) nifA gene upstream and downstream of the bleomycin resistance gene. This component was used in the project to edit the nitrogen-fixing bacteria (A. caul) genome by genetic recombination methods.[1] [1]Ryu, M. H., Zhang, J., Toth, T., Khokhani, D., Geddes, B. A., Mus, F., Garcia-Costas, A., Peters, J. W., Poole, P. S., Ané, J. M., & Voigt, C. A. (2020). Control of nitrogen fixation in bacteria that associate with cereals. Nature microbiology, 5(2), 314–330. https://doi.org/10.1038/s41564-019-0631-2

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 434
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1427
Illegal BamHI site found at 1766
Illegal XhoI site found at 547
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 234
Illegal NgoMIV site found at 339
Illegal NgoMIV site found at 556
Illegal NgoMIV site found at 1179
Illegal NgoMIV site found at 1240
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 331
Illegal BsaI.rc site found at 1287

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4115041 short</partinfo>
+Add the upstream and downstream fragments of the nitrogen-fixing bacteria (A. caul) nifA gene upstream and downstream of the bleomycin resistance gene. This component was used in the project to edit the nitrogen-fixing bacteria (A. caul) genome by genetic recombination methods.[1]
-none
+[1]Ryu, M. H., Zhang, J., Toth, T., Khokhani, D., Geddes, B. A., Mus, F., Garcia-Costas, A., Peters, J. W., Poole, P. S., Ané, J. M., & Voigt, C. A. (2020). Control of nitrogen fixation in bacteria that associate with cereals. Nature microbiology, 5(2), 314–330. https://doi.org/10.1038/s41564-019-0631-2
 <!-- Add more about the biology of this part here