Difference between revisions of "Part:BBa K4212036:Design"

 
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===Design Notes===
 
===Design Notes===
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x
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This part consists the chimera of CotG and ChiS, in which we combined these parts into level 1 Golden Gate Construct. Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. We added a flexible linker to this construct to test if it works better than the construct without the linker. We tried out different linkers to compare their effectiveness.Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. The addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG) can induce gene expression under the control of the promoter hyperspank. tL3S2P21 is a strong promoter, which helps to increase the production of desired protein.
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===Source===
 
===Source===
  
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Synthetic construct.
  
 
===References===
 
===References===
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https://parts.igem.org/Part:BBa_K143016

Revision as of 07:58, 4 October 2022


ChiS3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1912
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1912
    Illegal NheI site found at 1011
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1912
    Illegal BamHI site found at 1897
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1912
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1912
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part consists the chimera of CotG and ChiS, in which we combined these parts into level 1 Golden Gate Construct. Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. We added a flexible linker to this construct to test if it works better than the construct without the linker. We tried out different linkers to compare their effectiveness.Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams. The addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG) can induce gene expression under the control of the promoter hyperspank. tL3S2P21 is a strong promoter, which helps to increase the production of desired protein.



Source

Synthetic construct.

References

https://parts.igem.org/Part:BBa_K143016