Difference between revisions of "Part:BBa K4212034:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
x
+
This part consists the chimera of CotG and ChiS, in which we combined these parts into level 1 Golden Gate Construct. Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams.
  
  

Revision as of 07:39, 4 October 2022


ChiS1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1867
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1867
    Illegal NheI site found at 1011
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1867
    Illegal BamHI site found at 1852
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1867
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1867
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part consists the chimera of CotG and ChiS, in which we combined these parts into level 1 Golden Gate Construct. Our dry lab team predicted the functional model of this chimera and our wet lab intended to test the effectiveness of this model. Promoter hyper-spank is an inducible promoter, which was designed for high-level protein expression in B.subtilis and it was previously used by other iGEM teams.


Source

x

References