Difference between revisions of "Part:BBa K4463007"
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CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of ShMT3, a protein belongs to Metallothionein family, achieved by linking to csgA. SUMO tag was added to improve the solubility of recombinant protein, csgA and ShMT3. Improved parts is based on part BBa_K4463006.
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CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of ShMT3, a protein belongs to Metallothionein family, achieved by linking to csgA. SUMO tag was added to improve the solubility of recombinant protein, csgA and ShMT3. Improved parts is based on part <a href="http://https://parts.igem.org/Part:BBa_K4463006">BBa_K4463006</a>.
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The project of IGEM_XJTLU 2023 tested the adsorbed Cu(II) and Cd(II) concentration of part BBa_K4463006 and the part in this page.
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<img src="https://static.igem.wiki/teams/4463/wiki/figures-for-engineering-and-parts/shmt-adsorb-cu-3plasmids.png
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Figure1. The results showed that the ability of adsorbing Cu(II) is: SUMO-ShMT3> ShMT3> Control
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<img src="https://static.igem.wiki/teams/4463/wiki/figures-for-engineering-and-parts/shmt-adsorb-cd.png
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Figure2. The results showed that the ability of adsorbing Cd(II) is: SUMO-ShMT3> ShMT3> Control
  
  

Revision as of 07:16, 4 October 2022


SUMO-CsgA-ShMT3


CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of ShMT3, a protein belongs to Metallothionein family, achieved by linking to csgA. SUMO tag was added to improve the solubility of recombinant protein, csgA and ShMT3. Improved parts is based on part BBa_K4463006.

The project of IGEM_XJTLU 2023 tested the adsorbed Cu(II) and Cd(II) concentration of part BBa_K4463006 and the part in this page.

Figure1. The results showed that the ability of adsorbing Cu(II) is: SUMO-ShMT3> ShMT3> Control
Figure2. The results showed that the ability of adsorbing Cd(II) is: SUMO-ShMT3> ShMT3> Control Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]